REDUCE STATUS EFFECT DURATIONS BY 50 FREE

Glycogen was measured using a colorimetric procedure as previously described. Additionally, the heart, kidney, liver, visceral fat and skeletal muscle from the medial red gastrocnemius were rapidly removed and stored at -70℃ until analysis. All blood samples were immediately centrifuged (1300 RCF for 20 min at 4℃) to separate the plasma and erythrocytes. Immediately following decapitation, blood samples were collected in heparinized tubes (BD Vacutainer®). At each of the respective time points, the animals were sacrificed by decapitation while under light ether anesthesia. The three DE groups were sacrificed immediately after exercising on a treadmill (15° incline, 0.5-0.8 km/h) for 30 min (DE-0.5), 1 h (DE-1), or 2h (DE-2), respectively. The BE groups were sacrificed without having performed exercise at the end of week 4.

At the end of week 6, the animals were subdivided into four groups (five rats per group) based on exercise: before exercise (BE, n = 5) and during exercise (DE-0.5, n = 5 DE-1, n = 5 DE-2, n = 5). Rats received light electric shocks if they did not comply with running on the treadmill. For physical training, the rats exercised on a treadmill (Jungdo Bio & Plant, JD-A-09, Korea, 30 min/d, 15° incline, 0.5-0.8 km/h) 5 days per week. Therefore, this study was conducted to examine the influence of different durations of exercise on fuel utilization, lactate levels and antioxidant defense system in trained rats.įorty animals underwent either physical training (T, n = 20) or were sedentary (NT, n = 20) for 6 weeks.

REDUCE STATUS EFFECT DURATIONS BY 50 FREE

There is also accumulating evidence that strenuous exercise induces negative effects between free radical production and the body's antioxidant defense systems. At the skeletal muscle level, such training in humans is able to reduce the release of lactate from contracting muscle, mainly by improving mitochondrial respiratory capacity. As an adaptive response, antioxidant defense systems are up regulated and physical training shifts the maximum lactate steady state to a higher intensity. When the body is involved in physical training, certain metabolic processes occur to assure that adequate energy is provided to the exercising muscles via increased oxidation of fat and conservation carbohydrate storage. However, the effects of training in the state of different durations of aerobic exercise have not been fully elucidated. A number of mechanisms have been investigated to explain the physiological effects of aerobic exercise on fuel utilization, antioxidant defense system and lactate levels. The positive effects of physical training on animals have been generally accepted. Keywords: Duration of exercise, physical training, energy source, antioxidative system, lactate Overall, the results of this study, suggest that training may improve exercise performance by facilitating the mobilization and oxidation of fat and conserving limited carbohydrate storage, and that it may delay the onset of fatigue and enhance the antioxidative defense system, but cannot support two hours of vigorous exercise. There were no differences in MDA levels in BE and DE-0.5, but they were significantly lower in DE-1 and DE-2 of the T group. SOD activities were higher in trained rats, while the GSH/GSSG ratios were higher in BE, DE-0.5 and DE-1 in the T group, and there was no difference in that of DE-2. Catalase activity of the T group was lower in BE but higher in DE-0.5, DE-1 and DE-2.

Lactate levels were increased in DE-0.5 of the NT group, while they were increased in DE-1 of the T group. FFA levels were increased in DE-0.5 of the T group and in DE-1 of the NT group. Muscle TG levels were decreased in DE-0.5 of the T group, while those of the NT group were decreased in DE-1. Liver protein in DE-2 of the NT group was significantly lower. Muscle and liver glycogen levels were significantly lower in DE-1 and DE-2, but there were no significant differences between DE-1 and DE-2 in the T group. The plasma glucose level in DE-2 of the NT group was significantly lower than in the other groups. At the end of week 6, the animals in each group were subdivided into BE, DE-0.5, DE-1 and DE-2, which were sacrificed at the end of week 6 without having performed exercise or after exercise on a treadmill for 0.5h, 1h and 2h, respectively, immediately before being sacrificed. For physical training, animals exercised on a treadmill for 30 min 5 days per week. Forty rats underwent physical training (T, n = 20) or non- training (NT, n = 20) for 6 weeks. This study investigated the influence of different durations of aerobic exercise on fuel utilization, lactate levels and antioxidative status in trained rats.

Reduce status effect durations by 50